Von Willebrand Factor (vWF) BioAssay™ ELISA Kit (Canine)

Kits and Assays / Kits-ELISA (Coagulation Factors), BioAssay™

4°C/-20°C

Von Willebrand Factor (vWF) BioAssay™ ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of vWF in canine plasma.

Detection Range:
9.88-800ng/ml

Sensitivity:
4.11ng/ml

Precision:
Intra-Assay CV: <10%
Inter-Assay CV: <12%

Kit Components:
*028913A: Microtiter Strips, 1x48 wells or 1x96 wells
*028913B: Standard, 2x1 vial
028913C: Standard Diluent, 1x10ml or 1x20ml
*028913D: Detection Reagent A (green), 1x1 vial
*028913E: Detection Reagent B (red), 1x60ul or 1x120ul
028913F: Assay Diluent A, 1x6ml or 1x12ml
028913G: Assay Diluent B, 1x6ml or 1x12ml
028913H: TMB Substrate, 1x4.5ml or 1x9ml
028913K: Stop Solution, 1x3ml or 1x6ml
028913L: Wash Buffer, 30X, 1x10ml or 1x20ml
028913M: Detection Reagent A Diluent for 028913D, 1x300ul

Precaution:
The Stop Solution (028913K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability:
Store *028913A, *028913B, *028913D and *028913E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary:
1. Prepare all reagents, samples and standards.
2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C.
3. Aspirate and wash 3 times.
4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
5. Aspirate and wash 5 times.
6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37°C.
7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.