Caspase 1 (CASP1) Recombinant, Mouse

Blue Ice

21, 6

Supplied as lyophilized powder from PBS, pH 7.4, 5% trehalose, 0.05% sarcosyl. Reconstitute in sterile ddH2O to a concentration of 0.5mg/ml. Do not vortex.

Recombinant protein corresponding to aa119-296 with an N-Terminal His-Tag from mouse CASP1, expressed in E. coli. Swiss/UniProt Accession: P29452

Amino Acid Sequence:
NK EDGTFPGLTG TLKFCPLEKA QKLWKENPSE IYPIMNTTTR TRLALIICNT EFQHLSPRVG AQVDLREMKL LLEDLGYTVK VKENLTALEM VKEVKEFAAC PEHKTSDSTF LVFMSHGIQE GICGTTYSNE VSDILKVDTI FQMMNTLKCP SLKDKPKVII IQACRGEKQG VVLLKD

Molecular Weight:
21.6kD

Applications:
Suitable for use in ELISA, Western Blot, and SDS-PAGE.
Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Predicted Isoelectric Point:
6.8

Predicted Molecular Weight:
21.6

Accurate Molecular Weight:
23kD as determined by SDS-PAGE reducing conditions

Subcellular Location:
Cytoplasm, Chromosome

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.