Interferon Beta (IFNb) Recombinant, Canine

Blue Ice

52, 1

Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 5% trehalose, 0.01% sarcosyl. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0 to a concentration of 0.1-1mg/ml. Do not vortex.

Recombinant protein corresponding to Ala21~Asn186 with an N-Terminal His and GST Tag of canine IFNb expressed in E. coli Swiss/UniProt Accession: B6E116.

Predicted Isoelectric Point:
6.2

Predicted Molecular Weight:
50.1kD

Accurate Molecular Mass:
47kD as determined by SDS-PAGE under reducing conditions

Subcellular Location:
Secreted

Purity:
≥95%

Amino Acid Sequence:
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- AMSNDLLRSQ LSSSSLECQE LLLQLNGTTE YCLKDRINFE IPEEIEKSRQ FQKEDIILIT HEMFQKIFDI FRRNISRTGW NETTVENLLV KLHWQKEHLE IILEDVKEKE NFTWDNRTLL HLKKYYLRIV QYLKAKEYSI CAWTIVQAEI CRNFFFLNIL TDYLQN


Applications:
Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile sterile 20mM Tris, 150mM sodium chloride, pH 8.0. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note:
Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.