Aldolase B, Fructose Bisphosphate, Recombinant (ALDOB, ALDO-BL, Liver-type Aldolase)

Blue Ice

40, 6

Supplied as lyophilized powder in PBS, pH 7.4, 5% trehalose, 0.01% sarcosyl. Reconstitute with sterile PBS, pH 7.3.

Source:
E. coli

Molecular Weight:
~40.6kD

Endotoxin:
<1.0EU/1ug (LAL method)

Residues:
aa9-364 with two N-terminal Tags, His-tag and T7-tag

AA Sequence:
IA QSIVANGKGI LAADESVGTM GNRLQRIKVE NTEENRRQFR EILFSVDSSI NQSIGGVILF HETLYQKDSQ GKLFRNILKE KGIVVGIKLD QGGAPLAGTN KETTIQGLDG LSERCAQYKK DGVDFGKWRA VLRIADQCPS SLAIQENANA LARYASICQQ NGLVPIVEPE VIPDGDHDLE HCQYVTEKVL AAVYKALNDH HVYLEGTLLK PNMVTAGHAC TKKYTPEQVA MATVTALHRT VPAAVPGICF LSGGMSEEDA TLNLNAINLC PLPKPWKLSF SYGRALQASA LAAWGGKAAN KEATQEAFMK RAMANCQAAK GQYVHTGSSG AASTQSLFTA CYTY

Applications:
Suitable for use in SDS-PAGE, ELISA, Western Blot, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.