MAP1LC3B (Microtubule-associated Proteins 1A/1B light chain 3B, Autophagy-related Protein LC3 B/, Autophagy-related Ubiquitin-like Modifier LC3 B, MAP1 Light Chain 3-like Protein 2, MAP1A/MAP1B Light Chain 3 B, MAP1A/MAP1B LC3 B)

Antibodies / Antibodies-Microtubule-associated Proteins

-20°C

Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.

Synthetic peptide corresponding to residues in human MAP1LC3B.

MAP1LC3B, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3 beta, the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22aa from newly synthesized LC3 beta, followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3 may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry, Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:50-1:200
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:50- 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.