PIGF (Phosphatidyl Inositol-glycan Biosynthesis Class F Protein, PIG-F, GPI11 Homolog)

Antibodies / Antibodies-Proteins

-20°C

Supplied as a liquid in PBS, pH 7.3, 0.05% sodium azide.

Synthetic peptide corresponding to human PIGF.

Phosphatidyl inositol-glycans(PIGs) are multi-pass transmembrane proteins that localize to the endoplasmic reticulum. PIGs exhibit various functions but all are crucial for the biosynthesis of the glycosylphosphatidyl inositol (GPI) anchor. Some PIG proteins are components of the GPI transamidase complex and play a role in the recognition of either the GPI attachment signal or the lipid portion of GPI. Other PIGs belong to the glycosyltransferase complex and function in the transfer of N-acetyl glucosamine (GlcNAc) to phosphatidyl inositol (PI). A variety of other PIGs play distinct roles in GPI synthesis. PIG-F functions as an auxiliary subunit of ethanolamine phosphate (EtNP) transferases. It associates with PIG-O and is required for its expression and stability. Together these two PIGs function as an EtNP transferase and catalyze the transfer of EtNP to the third mannose (Man-3) of GPI. A mutation in the gene encoding PIG-F may result in a block of EtNP addition to Man-3 and lead to an absence of GPI-anchored proteins.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.