SARS (Serine-tRNA Ligase, Cytoplasmic, Seryl-tRNA Synthetase, SerRS, Seryl-tRNA(Ser/Sec) Synthetase, SERS)

Antibodies / Antibodies-Infectious Disease SARS

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.

Recombinant protein corresponding to human SARS.

Severe acute respiratory syndrome (SARS) coronavirus, a recently emergent infectious agent, shares little homology with previously known coronaviruses with respect to its genes and encoding proteins. Following this pattern, SARS nucleocapsid protein (SARS NC) only weakly resembles analogous proteins of the coronavirus family, and thus estimations of its function prove difficult. In fact, the region encoding SARS NC and other matrix and nucleocapsid proteins more closely resembles avian coronaviruses, while phylogenetic analyses indicate a mammalian origin for the replicase protein. SARS NC expression increases the binding of transcription factors to promotor sequences of c-Fos, ATF2, CREB-1, and FosB, all components of the AP-1 signaling pathway. Other signaling pathways, e.g. the NFkB pathway, are not affected, however, implying that pathway activation by SARS NC is selective, and thus possibly an important target in SARS functional studies.Most viral particles show the appearance of spike-like glycoprotein surface projections, giving rise to the virus' name, which is Latin for crown. These transmembrane spike glycoproteins protrude 20 nanometers from the surface.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:50-1:200
Western Blot: 1:500-1:2000
Immunohistochemistry: 1:50-1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.