CYP3A4

Pab

Blue Ice

Affinity Purified

Cytochrome P450 3A(CYP3A) belongs to the cytochrome P450 family and the enzymes constitute an important detoxification system that contributes to primary metabolism of more than half of all prescribed medications. CYP3A expression determines impairment of drug absorption and efficient systemic clearance in a tissue- specific manner (PMID:17975676).This antibody is specific to CYP3A4.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking, overnight.
Immunohistochemistry: 1:50-1:200
Immunofluorescence (IF-IC): 1:100-1:500
ELISA (peptide): 1:20, 000-1:40, 000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.