Agarose Low EEO (MB)

Molecular Biology / MB-BioSep Agarose

RT

White, homogeeous free flowing powder

Agarose Low EEO Molecular Biology Grade is manufactured from selected algae and harvested during specific periods of growth to produce gels that are easier to prepare than the equivalent polyacrylamide gels. Low EEO Agarose is recommended for a wide array of molecular biology procedures and offers enhanced resolution ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.

Applications:
• Analytical DNA Gels ≥ 1000bp
• Blotting Assays (Southern, northern)
• Radial Immunodiffusion
• Restriction Enzyme digestion products
• For use with all buffer systems

Recommended Usage (0.5X TBE):
ConcentrationMolecular Weight
1% 500bp-10kb
1.2% 350bp-7kb
1.5% 250bp-2kb

Appearance:
White, homogeneous, free flowing powder

EEO (pH 8.4, Wieme Method):
0.09-0.13

Loss on Drying:
≤7.0%

Melting Point(1.5%):
86.5-89.5°C

Gel Point (1.5%):
34.5-37.5°C

Gel Strength (1.5%):
≥2500g/cm2

Sulfate:
≤0.20%

Ash:
≤0.50%

Clarity (1.5%):
≤40 NTU

pH, Solution:
7.0 ± 0.6

pH, Gel:
7.0 ± 0.6

Absorbance (450nm):
As Reported

DNase and RNase:
None Detected

Quality Control:
Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.

Functional and Molecular Biology Testing:
To Pass Test:
• Comparative assay of different size DNA fragments
• Southern Blot (125-23, 000bp)
• Background Fluorescence (TAE/TE, EtBr):
• DNA Binding (TAE, EtBr)
• Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)

Storage and Stability:
Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.