Agarose Med EEO

RT

Molecular Biology Grade

Med EEO Agarose is a general use agarose suitable for many nucleic acid and protein electrophoresis protocols.

Applications:
Suitable for use in Immunoelectrophoresis, Serum protein electrophoresis, Analytical DNA Gels, 1000bp, Blotting Assays (Southern, northern), and with all buffer systems

Appearance:
White, granular, free flowing

pH (1.5%, Gel):
7.0±0.6

EEO (pH 8.4, Wieme method):
0.16-0.19

Loss on Drying:
≤7.0%

Melting Point (1.5%):
86.5-89.5°C

Gel Point (1.5%):
34.5-37.5°C

Gel Strength (1.5%):
≥2000g/cm2

Sulfate:
≤0.25%

Ash:
≤0.50%

Clarity (1.5%):
≤40NTU

Colorimetry (1.5%):
As Reported

RNase:
None Detected

DNase:
None Detected

Quality Control:
Genetic tested for a wide array of molecular biology procedures ensuring high recovery of biologically active DNA, high cloning efficiency and consistent reproducibility.

Functional and Molecular Biology Testing: To Pass Test
• Comparative assay of different size DNA fragments
• Southern Blot (125-23, 000bp)
• Background Fluorescence (TAE/TE, EtBr):
• DNA Binding (TAE, EtBr)
• Restriction Ligation Assay (Bam HI, Hinc II, EcoR I, Hind III, Pst I, and T4 DNA Ligase)

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Storage and Stability:
Store powdered agarose at RT. Opened bottles should be capped tightly and kept in a low humidity environment. For greater separation capacity, keep the gel at 4°C for 1 hour before use.