DNA (Deoxyribonucleic Acid), Sodium Salt (Calf Thymus)

Molecular Biology / MB-Cloning-Nucleic Acids

4°C/-20°C

Supplied as a highly purified dried powder

Specially prepared to be low in protein content but highly polymerized. Suitable as a substrate for deoxyribonuclease. This DNA is an excellent substrate for DNase. A sodium salt, it must be converted by adding magnesium ions to be susceptible to DNase.

• Low protein and RNA content
• Characterized by a high polymerization factor

Source:
Calf thymus isolated by the method established by Marmur.

Form:
Supplied as a highly purified dried powder

Hyperchromicity:
≥27%

Absorbance Ratio (A260/A280):
≥1.8

Recommended Storage Buffer:
10mM Tris-HCl. pH 8 containing 1mM EDTA (TE Buffer)

Quality Control:
SDS-PAGE

Storage and Stability:
Lyophilized powder may be stored at 4°C. Stable for 12 months at 4°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.