Nocodazole Solution in DMSO, 5mg/ml

RT

301, 32

Supplied as a liquid in DMSO.

Nocodazole, an inhibitor of microtubule formation, was found to greatly inhibit cell division around the mound stage as well as during the vegetative growth phase, when applied to exponentially growing Ax-2 cells. Mitotic arrest of cells with nocodazole can be used to study phosphorylation of microtubule-associated proteins which increase during mitosis, concomitant with increased microtubule dynamics. Nocodazole has been found to dramatically inhibit T cell antigen receptor (TCR) signaling, independently of its ability to depolymerize microtubules. Nocodazole arrests the cell cycle at G2/M phase and prevents phosphorylation of the T cell antigen receptor and inhibits its activity.

Synonyms:
Methyl N-(5-thenoyl-2-benzimidazolyl)carbamate; Methyl [5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl]carbamate; Oncodazole; R 17934; [5-(2-Thienylcarbonyl)-1H-benzimidazol-2-yl]-carbamic acid methyl ester

CAS No (dry powder):
31430-18-9

Molecular Formula:
C14H11N3O3S

Molecular Weight (dry powder):
301.32

Source:
Synthetic

Purity:
Nocodazole: ≥99% (HPLC); DMSO: ≥99.9%

Appearance:
Supplied as a liquid in DMSO.

Method for Determining Identity:
Proton NMR Spectroscopic Analysis

Concentration:
5mg/ml

Usage:
Use stock solution at 5mg/ml in DMSO. Further dilutions can be made in culture media. Due to insolubility (nocodazole is only slightly soluble in water), no more than 10ug/ml concentration can be used in cell culture systems. Nocodazole is stable in biological media for seven days.

Storage and Stability:
May be stored at RT for short-term only. Long-term storage is recommended at 4°C for 6 months after receipt.