Purification Media, Heparin Beads, BioSeparation™

Molecular Biology / MB-BioSep Affinity Beads

4°C Do not freeze

Supplied as a liquid in PBS pH 7.4, 0.3N sodium chloride added with 0.01% thimerosal as a preservative.

Heparin Beads are made by coupling 20-35kD size Heparin extracted from Porcine intestinal mucosa (Type I) to Sepharose beads. These beads are useful for isolating heparin-binding protein such as Antithrombin III, lipoproteins, various enzymes and DNA binding proteins.

Heparin Beads Specifications:

Matrix: Sepharose CL-4B

Coupling Method: TargetLock

Heparin Density: 0.7-1.2mg/ml of 20-35kD Heparin. Thrombin binding capacity minimum 0.7mg/ml of beads

Mean Bead Size: 40 -165um

Range of pH: 3.0-11.0

Bead Structure: Highly cross-linked spherical agarose, 4%

Max Back Pressure: 0.3MPa, 3 bar

Max. Flow Rates: 4ml/min/cm2

Recommended Flow Rate: 1-3ml/min/cm2

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.