Human Protein-tyrosine kinase 2-beta (PTK2B) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Protein-tyrosine kinase 2-beta microplate
Human Protein-tyrosine kinase 2-beta standard
Human Protein-tyrosine kinase 2-beta detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Protein tyrosine kinase 2 beta is a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2.

PTK2B; CADTK; CAKB; FADK2; FAK2; PKB; PTK; PYK2; RAFTK; CAK beta; calcium-dependent tyrosine kinase; cell adhesion kinase beta; focal adhesion kinase 2; proline-rich tyrosine kinase 2; protein kinase B; protein