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Human Mitogen-activated protein kinase 12 (MAPK12) ELISA Kit

Human Mitogen-activated protein kinase 12 (MAPK12) ELISA Kit

ArtNr	ABK-KTE61735-96Tx50
Hersteller	Abbkine Scientific
Menge	96 T x 50
Quantity options	48 T 96 T 96 T x 5 96 T x 50
Kategorie	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	MAPK12,ERK3,ERK6,P38GAMMA,PRKM12,SAPK-3,SAPK3,mitogen-activated protein kinase 3,stress-activated protein kinase 3
Versandbedingung	Gekühlt
Lieferbar
Manufacturer - Type	ELISA
Manufacturer - Applications	This Human Mitogen-activated protein kinase 12 (MAPK12) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK12 in samples. An antibody specific for MAPK12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category	Protein
Manufacturer - Conjugate / Tag	MAPK12
Shipping Temperature	Gel pack with blue ice.
Storage Conditions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background	Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. The protein encoded by this gene functions as a signal transducer during differentiation of myoblasts to myotubes.By screening with sequences based on those of the rat ERK3 gene, Lechner et al. (1996) isolated an ERK6 clone from a human skeletal muscle cDNA library. They reported that ERK6 appears to function as a signal transducer during differentiation of myoblasts to myotubes. Li et al. (1996) stated that SAPK3 is probably identical to the ERK6 gene cloned by Lechner et al. (1996).
Description	This Human Mitogen-activated protein kinase 12 (MAPK12) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK12.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits	Human Mitogen-activated protein kinase 12 (MAPK12) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPK12. No significant cross-reactivity or interference between Human MAPK12 and analogues was observed.
Usage Notes	• Do not mix components from different kit lots or use reagents beyond the kit expiration date. • Allow all reagents to warm to room temperature for at least 30 minutes before opening. • Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. • Unused wells must be kept desiccated at 4 °C in the sealed bag provided. • Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. • It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components	• Human Mitogen-activated protein kinase 12 microplate • Human Mitogen-activated protein kinase 12 standard • Human Mitogen-activated protein kinase 12 detect antibody • Streptavidin-HRP • Standard diluent • Assay buffer • HRP substrate • Stop solution • Wash buffer • Plate covers
Limit of detection	Please inquire
Assay duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type	Sandwich ELISA (quantitative)
Calibration range	Please inquire
Detection method	Colorimetric
Sample type	Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment	ELISA Testing
Manufacturer - Search Terms	MAPK12, ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3, mitogen-activated protein kinase 3, stress-activated protein kinase 3

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 96 T x 50

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