Human Mitogen-activated protein kinase 12 (MAPK12) ELISA Kit

This Human Mitogen-activated protein kinase 12 (MAPK12) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK12 in samples. An antibody specific for MAPK12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK12 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK12 bound in the initial step. The color development is stopped and the intensity of the color is measured.

MAPK12

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

This Human Mitogen-activated protein kinase 12 (MAPK12) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK12.

Human Mitogen-activated protein kinase 12 (MAPK12) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPK12. No significant cross-reactivity or interference between Human MAPK12 and analogues was observed.

• Human Mitogen-activated protein kinase 12 microplate
• Human Mitogen-activated protein kinase 12 standard
• Human Mitogen-activated protein kinase 12 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Please inquire

Cell culture supernatants, Serum, Plasma, Other biological fluids

MAPK12, ERK3, ERK6, P38GAMMA, PRKM12, SAPK-3, SAPK3, mitogen-activated protein kinase 3, stress-activated protein kinase 3