Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A (DYRK1A) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A microplate
Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A standard
Human Dual specificity tyrosine-phosphorylation-regulated kinase 1A detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

DYRK1A encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region.

DYRK1A; DYRK; DYRK1; HP86; MNB; MNBH; MNB/DYRK protein kinase; OTTHUMP00000109090; dual specificity YAK1-related kinase; mnb protein kinase homolog hp86; protein kinase minibrain homolog; serine/threonine kinase