Human Anti-alpha 1 adrenergic receptor IgG antibody (ADRA1-Ab-IgG) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96Tx50
ArtNr ABK-KTE62498-96Tx50
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human Anti-alpha 1 adrenergic receptor IgG antibody (ADRA1-Ab-IgG) ELISA Kit employs a two-site sandwich ELISA to quantitate ADRA1-Ab-IgG in samples. An antibody specific for ADRA1-Ab-IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADRA1-Ab-IgG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADRA1-Ab-IgG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRA1-Ab-IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Anti-alpha 1 adrenergic receptor IgG antibody microplate
Human Anti-alpha 1 adrenergic receptor IgG antibody standard
Human Anti-alpha 1 adrenergic receptor IgG antibody detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Anti-alpha 1 adrenergic receptor IgG antibody (ADRA1-Ab-IgG) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADRA1-Ab-IgG. No significant cross-reactivity or interference between Human ADRA1-Ab-IgG and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
The alpha 1c- Adrenergic Receptor, also known as alpha 1a-adrenoceptor, is activated by epinephrine and norepinephrine. This receptor mediates calcium release upon ligand binding. The alpha 1-c adrenoceptor is one of three subtypes of alpha 1-adrenergic receptor (alpha 1 c/a, alpha 1b, and alpha 1d). The alpha 1c receptor couples to phospholipase C via a GTP-binding protein insensitive to pertussis toxin. Three isoforms are generated by alternative splicing. However, the corresponding isoforms of the protein have similar ligand-binding properties, effector coupling, and tissue distribution. Expression of this receptor has been reported in prostate, liver, kidney, heart, brain, spinal cord, bladder, and various blood vessels. ESTs have been isolated from liver and testis libraries.
Alternative
ADRA1-Ab-IgG

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Menge: 96Tx50
Lieferbar: In stock
Listenpreis: 20.207,94 €
Preis: 20.207,94 €
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