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Mouse Mitochondrial peptide methionine sulfoxide reductase (MSRA) ELISA Kit

ArtNr ABK-KTE70936-96T
Hersteller Abbkine Scientific
Menge 96T
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Mouse Mitochondrial peptide methionine sulfoxide reductase (MSRA) ELISA Kit employs a two-site sandwich ELISA to quantitate MSRA in samples. An antibody specific for MSRA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMSRA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MSRA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MSRA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Mitochondrial peptide methionine sulfoxide reductase microplate
Mouse Mitochondrial peptide methionine sulfoxide reductase standard
Mouse Mitochondrial peptide methionine sulfoxide reductase detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Mitochondrial peptide methionine sulfoxide reductase (MSRA) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MSRA. No significant cross-reactivity or interference between Mouse MSRA and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
MSRA encodes a ubiquitous and highly conserved protein that carries out the enzymatic reduction of methionine sulfoxide to methionine. Human and animal studies have shown the highest levels of expression in kidney and nervous tissue. The methionine sulfoxide reductase A functions in the repair of oxidatively damaged proteins to restore biological activity. Alternative splicing results in multiple transcript variants.
Gene ID
110265
Alternative
MSRA; Cytosolic methionine-S-sulfoxide reductase; peptide met (O) reductase
Accession
Q9D6Y7

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96T
Lieferbar: In stock
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