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Mouse Doublesex- and mab-3-related transcription factor 1 (DMRT1) ELISA Kit

ArtNr ABK-KTE71242-96Tx50
Hersteller Abbkine Scientific
Menge 96Tx50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Mouse (Murine, Mus musculus)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Mouse Doublesex- and mab-3-related transcription factor 1 (DMRT1) ELISA Kit employs a two-site sandwich ELISA to quantitate DMRT1 in samples. An antibody specific for DMRT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Mouse Doublesex- and mab-3-related transcription factor 1 microplate
Mouse Doublesex- and mab-3-related transcription factor 1 standard
Mouse Doublesex- and mab-3-related transcription factor 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Mouse Doublesex- and mab-3-related transcription factor 1 (DMRT1) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DMRT1. No significant cross-reactivity or interference between Mouse DMRT1 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
DMRT1 is found in a cluster with two other members of the gene family, having in common a zinc finger-like DNA-binding motif (DM domain). The DM domain is an ancient, conserved component of the vertebrate sex-determining pathway that is also a key regulator of male development in flies and nematodes, and is found to be the key sex-determining factor in chickens. This gene exhibits a gonad-specific and sexually dimorphic expression pattern, just like the related doublesex gene in fruit flies. The protein contains a nuclear localization signal between the 2 zinc-binding sites of the DM domain, which is located in the N terminus. Immunohistochemical analysis of adult human testis revealed that DMRT1 localized to nuclei of Sertoli cells, spermatogonia, and spermatocytes.
Gene ID
50796
Alternative
DMRT1; DMT1; DM domain expressed in testis 1
Accession
Q9QZ59

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx50
Lieferbar: In stock
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