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Pig Transforming growth factor beta3 (TGF-beta3) ELISA Kit

ArtNr ABK-KTE80038-96Tx5
Hersteller Abbkine Scientific
Menge 96Tx5
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Porcine
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Pig Transforming growth factor beta3 (TGF-beta3) ELISA Kit employs a two-site sandwich ELISA to quantitate TGFB3 in samples. An antibody specific for TGFB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTGFB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFB3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Pig Transforming growth factor beta3 microplate
Pig Transforming growth factor beta3 standard
Pig Transforming growth factor beta3 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Pig Transforming growth factor beta3 (TGF-beta3) ELISA Kit has high sensitivity and excellent specificity for detection of Pig TGFB3. No significant cross-reactivity or interference between Pig TGFB3 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Transforming growth factor beta-3 is a protein that in humans is encoded by the TGFB3 gene. It is a type of protein, known as a cytokine, which is involved in cell differentiation, embryogenesis and development. It belongs to a large family of cytokines called the Transforming growth factor beta superfamily, which includes the TGF-beta family, Bone morphogenetic proteins (BMPs), growth and differentiation factors (GDFs), inhibins and activins. TGF-beta3 is believed to regulate molecules involved in cellular adhesion and extracellular matrix (ECM) formation during the process of palate development. Without TGF-beta3, mammals develop a deformity known as a cleft palate. This is caused by failure of epithelial cells in both sides of the developing palate to fuse. TGF-beta3 also plays an essential role in controlling the development of lungs in mammals, by also regulating cell adhesion and ECM formation in this tissue, and controls wound healing by regulating the movements of epidermal and dermal cells in injured skin.
Gene ID
397400
Alternative
TGFB3; ARVD; FLJ16571; TGF-beta3;
Accession
P15203

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx5
Lieferbar: In stock
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