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Pig Adiponectin receptor 1 (ADIPOR1) ELISA Kit

ArtNr ABK-KTE80220-96Tx50
Hersteller Abbkine Scientific
Menge 96Tx50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Porcine
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Pig Adiponectin receptor 1 (ADIPOR1) ELISA Kit employs a two-site sandwich ELISA to quantitate ADIPOR1 in samples. An antibody specific for ADIPOR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADIPOR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADIPOR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADIPOR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Pig Adiponectin receptor 1 microplate
Pig Adiponectin receptor 1 standard
Pig Adiponectin receptor 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Pig Adiponectin receptor 1 (ADIPOR1) ELISA Kit has high sensitivity and excellent specificity for detection of Pig ADIPOR1. No significant cross-reactivity or interference between Pig ADIPOR1 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Human ADIPOR1 also has 375 amino acids and shares 96.8% identity with the mouse protein. ADIPOR1 and ADIPOR2 are highly related structurally, and mouse Adipor1 and Adipor2 share 66.7% identity. ADIPOR1 and ADIPOR2 are 7-transmembrane domain proteins, but they are structurally, topologically, and functionally distinct from G protein-coupled receptors (GPCRs). Epitope tag labeling showed that the N terminus is internal and the C terminus is external in the ADIPORs, a topology opposite that of GPCRs. ADIPOR1 and ADIPOR2 are conserved from yeast to human, especially in the membrane-spanning regions. The authors noted that the yeast homolog has a principal role in metabolic pathways that regulate lipid metabolism, such as fatty acid oxidation.
Gene ID
51094
Alternative
ADIPOR1
Accession
Q96A54

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx50
Lieferbar: In stock
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