Rat S-adenosylmethionine synthase isoform type-2 (MAT2A) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Rat S-adenosylmethionine synthase isoform type-2 microplate
Rat S-adenosylmethionine synthase isoform type-2 standard
Rat S-adenosylmethionine synthase isoform type-2 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Methionine adenosyltransferase (EC 2.5.1.6) catalyzes the biosynthesis of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the major methyl donor for many of the transmethylation reactions in the body.Three forms of MAT have been identified in mammalian tissues. MAT I and MAT III, which are encoded by the single-copy MAT1A gene, represent tetramers and dimers, respectively, formed from identical alpha-1 subunits and are synthesized primarily in the liver. The MAT2A promoter is highly GC rich, especially in the first 300 bp. The promoter contains several potential SP1-, v-myb-, and GATA-binding sites, as well as potential binding sites for C/EBP, HSF2, c-myb, and STATx.MAT2A promoter can efficiently drive expression from a reporter gene in both Jurkat and 293 cells.

MAT2A; MATA2; MATII; SAMS2;