Vergleich

TUNEL Apoptosis Detection Kit (Green Fluorescence)

ArtNr ABK-KTA2010-100T
Hersteller Abbkine Scientific
Menge 100T
Kategorie
Typ Assay-Kit
Specific against other
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Lieferbar
Applications notes
Abbkine TUNEL Apoptosis Detection Kit (Green Fluorescence) relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein. This kit provides all the essential components with an optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected at the popular FITC channel ( Ex/Em = 490nm/520 nm).
Kit components

TdT Enzyme
Equilibration Buffer (5X)
Label Nucleotide Mix
Features & Benefits
Optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer.
Signal can be easily detected at the popular FITC channel (Ex/Em = 490nm/520 nm).
Usage notes
The reaction buffer contains cacodylate and cobalt chloride, highly toxic chemicals. After contact with skin, wash immediately with plenty of water. In case of accident or if you feel unwell, seek medical advice immediately. Do not drink, eat or smoke when using.
Storage instructions
Stable for at least 6 months at -20C from date of shipment. Once opened, refer to list of materials supplied for storage conditions of individual components.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3?-hydroxyl termini in the double-strand DNA breaks generated during apoptosis. The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100T
Lieferbar: In stock
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