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TUNEL Apoptosis Detection Kit (Green Fluorescence)

TUNEL Apoptosis Detection Kit (Green Fluorescence)

Manufacturer	Abbkine Scientific
Category	Cell Biology Cell Status Apoptosis Autophagy Cardiovascular Research Ischemia & Reperfusion Injury Other Assays & Kits Detection Kits
Type	Assay-Kit
Specific against	other
Amount	100T
Item no.	ABK-KTA2010-100T
eClass 6.1	32161000
eClass 9.0	32161090
Available
Applications notes	Abbkine TUNEL Apoptosis Detection Kit (Green Fluorescence) relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein. This kit provides all the essential components with an optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected at the popular FITC channel ( Ex/Em = 490nm/520 nm).
Kit components	TdT Enzyme Equilibration Buffer (5X) Label Nucleotide Mix
Features & Benefits	Optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Signal can be easily detected at the popular FITC channel (Ex/Em = 490nm/520 nm).
Usage notes	The reaction buffer contains cacodylate and cobalt chloride, highly toxic chemicals. After contact with skin, wash immediately with plenty of water. In case of accident or if you feel unwell, seek medical advice immediately. Do not drink, eat or smoke when using.
Storage instructions	Stable for at least 6 months at -20C from date of shipment. Once opened, refer to list of materials supplied for storage conditions of individual components.
Shipping	Gel pack with blue ice.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	One of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3?-hydroxyl termini in the double-strand DNA breaks generated during apoptosis. The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100T

available

Delivery expected until 7/18/2024