PurKine Antibody Purification Protein A Kit

PurKine Protein A Resin has been immobilized for use in IgG purification applications in gravity column procedures at a variety of scales. The Resin consists of 90um beads of cross-linked 4% agarose, to which Recombinant protein A has been coupled. Tests confirm that performance equals or exceeds popular Recombinant protein A resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.

High capacity—More than 40mg human lgG per mL of resin.

High performance—Native protein A has five IgG binding domains and many other domains with unknown functions.Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding .


Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.

Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
Easy to use— Pre-formulated buffers available for kit formats.

Blue ice

PurKine Protein A Purification system is designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Recombinant protein A is produced in E. coli and functions essentially the same as native Protein A from Staphylococcus aureus. The Protein A molecule contains four high-affinity binding sites capable of interacting with the Fc region from IgG of several species. Protein A is generally preferred for affinity purification of rabbit, Guinea pig, dog and cat IgG. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine Protein A Purification Resin is also available as prepacked spin column and kit formats.