PurKine Antibody Purification Protein G Kit

PurKine Protein G Resin has been immobilized for use in IgG purification applications in gravity column procedures at a variety of scales. The Resin consists of 90um beads of cross-linked 4% agarose, to which Recombinant protein G has been coupled. Tests confirm that performance equals or exceeds popular Recombinant protein G resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.

High capacity—More than 30mg human lgG per mL of resin.

High performance—Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding which have been eliminated from recombinant protein G to reduce nonspecific binding.

Cost-effective—No performance decrease after at least five repeated uses of the same batch of resin.
Flexible—available in multiple formats including bulk resin, spin columns and complete kits.
Easy to use— Pre-formulated buffers available for kit formats.
Easy to use— Pre-formulated buffers available for kit formats.

Blue ice

PurKine Protein G Purification system is effective for affinity purification of IgG from serum and other fluids of many mammalian species. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA. The portfolio allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine Protein G Purification Resin is also available as prepacked spin column and kit formats.