Recombinant Human IFN-gamma Protein

< 0.1 EU/μg of the protein by LAL method.

ice pack

16.78 kDa

Recombinant Human IFN-gamma Protein is produced by E. coli expression system. The target protein is expressed with sequence ( Gln24-Gln166) of human Interferon Gamma (Accession #NP_000610.2).

Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

No tag

Cytokines & Cytokine receptors, Cell Culture related, Biosimilar Drug Targets

1. Measured by its binding ability in a functional ELISA. Immobilized Human FNGR1/CD119 at 1 μg/mL (100 μL/well) can bind Human IFNG with a linear range of 0. 98-1. 97 ng/mL.|2. Measured in a cytotoxicity assay using HT-29 cells. The ED₅₀ for this effect is 0. 74-2. 96 ng/mL, corresponding to a specific activity of 3. 38×10⁵~1. 35×10⁶ units/mg. |3. Flow cytometry: 1X106 A549 cells treated with IFN-γ (RP01038, 1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human PD-L1/CD274 mAb (A26692, 5 μl/Test, right).4. Flow cytometry: 1X106 NCI-H460 cells treated with IFN-γ (RP01038, 1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human PD-L1/CD274 mAb (A26692, 5 μl/Test, right)

16.78 kDa