SVGp12 cells

Disease:

Normal (Immortalized)

Origin:

The SVG p12 cell line was established by transfecting cultured human fetal glial cells from brain material dissected from 8 to 12 week old embryos with DNA from an ori - mutant of SV40.

Species:

Homo sapiens, human

Tissue:

Brain

Morphology:

Fibroblast; SV40 transformed astroglia

Properties:

Adherent

Cytogenic data:

Not specified

Patient:

fetus, first trimester

Medium:

AddexBio-formulated EMEM (C0005-01) + 10% FBS

Subculture:

Split sub-confluent cultures (70-80%) at a seed ratio of 1:2, i.e. seeding at 5x10, 000 cells/cm^2 using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days.

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO