SVGp12 cells

Disease:
Normal (Immortalized)
Origin:
The SVG p12 cell line was established by transfecting cultured human fetal glial cells from brain material dissected from 8 to 12 week old embryos with DNA from an ori - mutant of SV40.
Species:
Homo sapiens, human
Tissue:
Brain
Morphology:
Fibroblast; SV40 transformed astroglia
Properties:
Adherent
Cytogenic data:
Not specified
Patient:
fetus, first trimester
Medium:
AddexBio-formulated EMEM (C0005-01) + 10% FBS
Subculture:
Split sub-confluent cultures (70-80%) at a seed ratio of 1:2, i.e. seeding at 5x10, 000 cells/cm^2 using 0.25% trypsin; 0.05% EDTA. Culture at 5% CO2, 37C. Renew medium once every 2 to 3 days.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
II [Cells contain polyomavirus DNA sequences]
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
DNA Profile (STR):
Amelogenin: X, Y
CSF1PO: 10, 11, 12
D13S317: 8, 13, 14
D16S539: 10, 11, 12
D5S818: 11, 12, 14
D7S820: 10, 12, 13
THO1: 6, 7, 9, 9.3
TPOX: 8, 10, 11
vWA: 15, 16, 18, 19
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: SVG p12 (AddexBio T0005008)

Cell Lines