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Sarcoma
C3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells.
Mouse (Mus musculus) C3H strain Tissue:Embryo Morphology:Fibroblast Properties: Adherent Cytogenic data:Mouse karyotype with a modal number of 80 chromosomes. Medium: MEM Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Embryo Morphology:Fibroblast Properties: Adherent Cytogenic data:Mouse karyotype with a modal number of 80 chromosomes. Medium: MEM Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Fibroblast Properties: Adherent Cytogenic data:Mouse karyotype with a modal number of 80 chromosomes. Medium: MEM Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Adherent Cytogenic data:Mouse karyotype with a modal number of 80 chromosomes. Medium: MEM Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Mouse karyotype with a modal number of 80 chromosomes. Medium: MEM Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
MEM Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Complete culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Hepatitis B: Negative Hepatitis C: Negative
Hepatitis C: Negative
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