C3H/10T1/2 Clone 8 cells

Disease:
Sarcoma
Origin:
C3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells.
Species:
Mouse (Mus musculus) C3H strain
Tissue:
Embryo
Morphology:
Fibroblast
Properties:
Adherent
Cytogenic data:
Mouse karyotype with a modal number of 80 chromosomes.
Medium:
MEM Medium + 10% FBS
Subculture:
When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C.
Freezing Medium:
Complete culture medium supplemented with 5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Hepatitis C: Negative

Cell Lines