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Sarcoma
C3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells.
Mouse (Mus musculus) C3H strain Tissue:Embryo Morphology:Fibroblast Properties: Adherent Cytogenic data:Mouse karyotype with a modal number of 80 chromosomes. Medium: MEM Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Embryo Morphology:Fibroblast Properties: Adherent Cytogenic data:Mouse karyotype with a modal number of 80 chromosomes. Medium: MEM Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Fibroblast Properties: Adherent Cytogenic data:Mouse karyotype with a modal number of 80 chromosomes. Medium: MEM Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Adherent Cytogenic data:Mouse karyotype with a modal number of 80 chromosomes. Medium: MEM Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Mouse karyotype with a modal number of 80 chromosomes. Medium: MEM Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
MEM Medium + 10% FBS Subculture:When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
When confluent using 0.25% trypsin or trypsin/EDTA, subculture at 2000 viable cells/cm2; feed cells 2 to 3 times weekly. Culture at 5% CO2, 37C. Freezing Medium:Complete culture medium supplemented with 5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Complete culture medium supplemented with 5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
HIV: Negative Hepatitis B: Negative Hepatitis C: Negative
Hepatitis B: Negative Hepatitis C: Negative
Hepatitis C: Negative
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.
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