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U-251MG cells

U-251MG cells

Item no.	C0005029
Manufacturer	Addexbio
Amount	One Frozen vial
Category	Cell Lines & Cell Culture Cell Lines
Type	Tumour Cells
Specific against	other
Dry ice	Yes
Citations	1. Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol Microbiol Scand. 1968;74(4):465-486. PMID: 4313504
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41106509
Available
Description	Disease: Human glioblastoma astrocytoma Origin: Derived from a malignant glioblastoma tumor by explant technique. Species: Homo sapiens, human Tissue: Brain Morphology: Pleomorphic/astrocytoid Properties: Adherent Cytogenic data: 2n = 46 Medium: AddexBio-formulated EMEM Medium (C0005-01) + 10% FBS Subculture: Subculture sub-confluent cultures (70-80%) at 1:3 to 1:6 ratio, i.e. seeding at 2-4x10, 000 cells/cm^2 using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C. Freezing Medium: Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative DNA Profile (STR): Amelogenin: X, Y CSF1PO: 11, 12 D13S317: 10, 11 D16S539: 12 D5S818:11, 12 D7S820: 10, 12 THO1: 9.3 TPOX: 8 vWA: 16, 18 Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: U-251MG (AddexBio C0005029)
Storage condition	LN2
Manufacturers Category	Cell Lines

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: One Frozen vial

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Delivery expected until 8/28/2025

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