H9c2(2-1) cells

Disease:
Normal myoblast
Origin:
H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle.
Species:
Rattus norvegicus, rat
Tissue:
Heart/myocardium
Morphology:
Myoblast
Properties:
Adherent
Strain:
BD1X embryo
Medium:
AddexBio-formulated DMEM (C0003-01) + 10% FBS
Subculture:
Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: H9c2(2-1) (AddexBio C0031002)

Cell Lines