H9c2(2-1) cells

Disease:

Normal myoblast

Origin:

H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle.

Species:

Rattus norvegicus, rat

Tissue:

Heart/myocardium

Morphology:

Myoblast

Properties:

Adherent

Strain:

BD1X embryo

Medium:

AddexBio-formulated DMEM (C0003-01) + 10% FBS

Subculture:

Subculture sub-confluent cultures (70-80%) at 1:2 to 1:4 ratio, using 0.25% (w/v) Trypsin- 0.53 mM EDTA solution. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO