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Pheochromocytoma
The PC-12 cell line was derived from a transplantable rat pheochromocytoma.
Rattus norvegicus, rat Tissue: Adrenal gland Morphology: Polygonal Properties: Adherent, small clusters Strain: Male Medium: F-12K + 2.5% FBS + 15% Horse Serum. Subculture: Add appropriate aliquots of the cell suspension to new Corning CellBIND 75 cm^2 flask with 15 mL fresh growth medium. Seed flask at 1.0 x 10^4 to 3.0 x 10^4 viable cells / cm^2 or use subcultivation ratio of 1:3 twice weekly. Subculture when cell density reaches between 1.0 x 10^5 to 2.0 x 10^5 viable cells/cm^2. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
Adrenal gland Morphology: Polygonal Properties: Adherent, small clusters Strain: Male Medium: F-12K + 2.5% FBS + 15% Horse Serum. Subculture: Add appropriate aliquots of the cell suspension to new Corning CellBIND 75 cm^2 flask with 15 mL fresh growth medium. Seed flask at 1.0 x 10^4 to 3.0 x 10^4 viable cells / cm^2 or use subcultivation ratio of 1:3 twice weekly. Subculture when cell density reaches between 1.0 x 10^5 to 2.0 x 10^5 viable cells/cm^2. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
Polygonal Properties: Adherent, small clusters Strain: Male Medium: F-12K + 2.5% FBS + 15% Horse Serum. Subculture: Add appropriate aliquots of the cell suspension to new Corning CellBIND 75 cm^2 flask with 15 mL fresh growth medium. Seed flask at 1.0 x 10^4 to 3.0 x 10^4 viable cells / cm^2 or use subcultivation ratio of 1:3 twice weekly. Subculture when cell density reaches between 1.0 x 10^5 to 2.0 x 10^5 viable cells/cm^2. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
Adherent, small clusters Strain: Male Medium: F-12K + 2.5% FBS + 15% Horse Serum. Subculture: Add appropriate aliquots of the cell suspension to new Corning CellBIND 75 cm^2 flask with 15 mL fresh growth medium. Seed flask at 1.0 x 10^4 to 3.0 x 10^4 viable cells / cm^2 or use subcultivation ratio of 1:3 twice weekly. Subculture when cell density reaches between 1.0 x 10^5 to 2.0 x 10^5 viable cells/cm^2. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
Male Medium: F-12K + 2.5% FBS + 15% Horse Serum. Subculture: Add appropriate aliquots of the cell suspension to new Corning CellBIND 75 cm^2 flask with 15 mL fresh growth medium. Seed flask at 1.0 x 10^4 to 3.0 x 10^4 viable cells / cm^2 or use subcultivation ratio of 1:3 twice weekly. Subculture when cell density reaches between 1.0 x 10^5 to 2.0 x 10^5 viable cells/cm^2. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
F-12K + 2.5% FBS + 15% Horse Serum. Subculture: Add appropriate aliquots of the cell suspension to new Corning CellBIND 75 cm^2 flask with 15 mL fresh growth medium. Seed flask at 1.0 x 10^4 to 3.0 x 10^4 viable cells / cm^2 or use subcultivation ratio of 1:3 twice weekly. Subculture when cell density reaches between 1.0 x 10^5 to 2.0 x 10^5 viable cells/cm^2. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
Add appropriate aliquots of the cell suspension to new Corning CellBIND 75 cm^2 flask with 15 mL fresh growth medium. Seed flask at 1.0 x 10^4 to 3.0 x 10^4 viable cells / cm^2 or use subcultivation ratio of 1:3 twice weekly. Subculture when cell density reaches between 1.0 x 10^5 to 2.0 x 10^5 viable cells/cm^2. Culture at 5% CO2, 37C. Freezing Medium:Complete growth culture medium supplemented with 7.5% (v/v) DMSO Biosafety Level: I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
I Sterility: Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
Bacteria: Negative Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
Yeast: Negative Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
Mycoplasma: Negative Pathogens: HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
HIV: Negative Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
Hepatitis B: Negative Citation Format: If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)
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C0032002-datasheet.pdf
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