PC-12adh cells

Disease:
Pheochromocytoma
Origin:
The PC-12 cell line was derived from a transplantable rat pheochromocytoma.
Species:
Rattus norvegicus, rat
Tissue:
Adrenal gland
Morphology:
Polygonal
Properties:
Adherent, small clusters
Strain:
Male
Medium:
F-12K + 2.5% FBS + 15% Horse Serum.
Subculture:
Add appropriate aliquots of the cell suspension to new Corning CellBIND 75 cm^2 flask with 15 mL fresh growth medium. Seed flask at 1.0 x 10^4 to 3.0 x 10^4 viable cells / cm^2 or use subcultivation ratio of 1:3 twice weekly. Subculture when cell density reaches between 1.0 x 10^5 to 2.0 x 10^5 viable cells/cm^2. Culture at 5% CO2, 37C.
Freezing Medium:
Complete growth culture medium supplemented with 7.5% (v/v) DMSO
Biosafety Level:
I
Sterility:
Bacteria: Negative
Yeast: Negative
Mycoplasma: Negative
Pathogens:
HIV: Negative
Hepatitis B: Negative
Citation Format:
If use of this culture results in a scientific publication, it should be cited in the publication as: PC-12Adh (AddexBio C0032002)

Cell Lines