PC-12adh cells

Disease:

Pheochromocytoma

Origin:

The PC-12 cell line was derived from a transplantable rat pheochromocytoma.

Species:

Rattus norvegicus, rat

Tissue:

Adrenal gland

Morphology:

Polygonal

Properties:

Adherent, small clusters

Strain:

Male

Medium:

F-12K + 2.5% FBS + 15% Horse Serum.

Subculture:

Add appropriate aliquots of the cell suspension to new Corning CellBIND 75 cm^2 flask with 15 mL fresh growth medium. Seed flask at 1.0 x 10^4 to 3.0 x 10^4 viable cells / cm^2 or use subcultivation ratio of 1:3 twice weekly. Subculture when cell density reaches between 1.0 x 10^5 to 2.0 x 10^5 viable cells/cm^2. Culture at 5% CO2, 37C.

Freezing Medium:

Complete growth culture medium supplemented with 7.5% (v/v) DMSO