Anti-Fibrinopeptide D

The reactivity of the antiserum is reacting with the fragment D as well as intact fibrinogen, fibrin monomers, fragment X, fragment Y and fragment D.D. In bidimensional electrophoresis against normal human one single precipitin line is obtained. No reaction is obtained with any other plasma protein or normal serum. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul in a 3 mm diameter center well and 2 ul serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.

Precipitating polyclonal Rabbit anti-Serum to Human fibrinopeptide D.

Fibrinogen (clotting factor I) is a heat labile beta glycoprotein (molecular weight 340, 000) and consists of three pairs of chains bound by disulphide bonds. It is synthesized in hepatocytes under genetic control. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis, releasing the fibrinopeptides A and B (molecular weight 50, 000-65, 000) and forming fibrin monomers. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking under the influence of factor XIII. The predominant gamma chain of normal fibrinogen (MW 50, 000, with higher variants) has a low affinity for platelet binding. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund's complete adjuvant is used in the first step of the immunization procedure.

For Research Use Only.