Anti-Leukocyte Associated Immunoglobulin Like Receptor 1 (LAIR1)

LAIR1 is a type I membrane protein with a leader sequence, an extracellular domain, a transmembrane domain, and a cytoplasmic region. The 142-amino acid extracellular domain contains 1 N-linked glycosylation site, and a pair of cysteines that generates a single immunoglobulin-like domain, thus classifying LAIR1 as a member of the immunoglobulin superfamily. The 101-amino acid cytoplasmic tail contains 2 ITIMs. The LAIR1 protein shares less than 30% homology with members of the inhibitory immunoglobulin superfamily and 32% homology with members of the immunoglobulin-like transcript family. Immunoprecipitation of LAIR1 from NK cells, followed by SDS-PAGE analysis, detected 2 proteins: an approximately 40-kD monomer, which decreased to approximately 32 kD after deglycosylation, and an approximately 32-kD protein.

Western blotting: 1:100-400, Immunocytochemistry in formalin fixed cells: 1:100-500, Immunohistochemistry in formalin fixed frozen section: 1:100-500, Immunohistochemistry in paraffin section: 1:50-417

CD305

Human