Anti-Heat Shock 70kDa Binding Protein 1 (HSPBP1)

Using the ATPase domain of human HSP70 as bait in a yeast 2-hybrid screen of a human heart cDNA library, Raynes and Guerriero (1998) cloned HSPBP1. The deduced 459-amino acid protein has a calculated molecular mass of 39.3 kD and contains 6 consecutive glycines near its N terminus. Northern blot analysis detected a 1.7-kb HSPBP1 transcript in all tissues examined, with highest expression in heart and skeletal muscle. Raynes and Guerriero (1998) also identified an HSPBP1 variant that they called HSPBP2, which has 2 polyadenylation signals rather than the single polyadenylation signal of HSPBP1. The deduced HSPBP2 protein is identical to HSPBP1 except that it has 9 consecutive glycines near the N terminus.

Western blotting: 1:100-400, Immunocytochemistry in formalin fixed cells: 1:100-500, Immunohistochemistry in formalin fixed frozen section: 1:100-500, Immunohistochemistry in paraffin section: 1:50-417

FES1, Hsp70 Interacting Protein, Hsp70 Binding Protein, Cytoplasmic Cochaperone 1

Human