Anti-Lecithin Cholesterol Acyltransferase (LCAT)

Lecithin-cholesterol acyltransferase is an enzyme that converts free cholesterol into cholesteryl ester (a more hydrophobic form of cholesterol), which is then sequestered into the core of a lipoprotein particle, eventually making the newly synthesized HDL spherical and forcing the reaction to become unidirectional since the particles are removed from the surface. The enzyme is bound to high-density lipoproteins (HDLs) and low-density lipoproteins in the blood plasma. An unusual feature of the message is that the poly(A) signal appears to overlap the COOH-terminal glutamic acid and stop codons. The protein has several extended sequences of hydrophobic amino acids, one of which is similar to sequences in pancreatic lipase and lingual lipase.

Western blotting: 1:100-400, Immunocytochemistry in formalin fixed cells: 1:100-500, Immunohistochemistry in formalin fixed frozen section: 1:100-500, Immunohistochemistry in paraffin section: 1:50-417

phosphatidylcholine-sterol O-acyltransferase

Rat