SensoLyte® Generic MMP Assay Kit Colorimetric - 1 kit

Protease activity

Matrix Metalloproteinase (MMP)

Store all components at -20°C. Store component C at 4°C and protect from the light. Component G can be at room temperature for convenience. Aliquot Components E and F as needed to avoid freeze-thaw cycles.

Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion as well as other diseases is well known.
The SensoLyte® Generic MMP Assay Kit can be used to detect the activity of a variety of MMPs, including MMP-1, 2, 3, 7, 8, 9, 12, 13, and 14 or for high throughput screening of MMPs’ inducers and inhibitors. The substrate is a thiopeptolide that is cleaved by the MMPs, releasing a sulfhydryl group, which can be detected with a color-developing thiol-reactive agent, 4, 4’-dithiodipyridine or Ellman’s Reagent at 412 nm using a microplate reader. The assays are performed in a convenient 96-well microplate format.

412

nanogram

Research use

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