Goat anti-PPP2CA / PPP2CB Antibody

Pep ELISA, WB

Goat anti-PPP2CA / PPP2CB Antibody
Principle Name: PPP2CA / PPP2CB
Official Symbol: PPP2CA / PPP2CB
All Name and Symbols: PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; PP2Ac; PP2CA; RP-C; PP2A-alpha; protein phosphatase 2, catalytic subunit, alpha isoform; replication protein C; serine/threonine protein phosphatase 2A, catalytic subunit, alph
Accession Number(s): NP_002706.1; NP_004147.1
Peptide Sequence: EPHVTRRTPDYFL, is from C Terminus
Human Gene ID(s): 5515; 5516
Non-Human GeneID(s): 19052 (mouse); 24672 (rat);
Important Comments: Two variants NP_001009552.1 and NP_004147.1 represent identical protein.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 36kDa band observed in Human Brain (Cerebellum), Mouse, Rat and Pig Brain lysates (calculated MW of 35.6kDa according to Human NP_002706.1, Mouse NP_062284.1, Rat NP_058735.1 and Pig NP_999531.1). Recommended concentration: 0.05-0.2
Reference(s): Hemmings BA, Wernet W, Mayer R, Maurer F, Hofsteenge J, Stone SR. The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A beta catalytic subunit. Nucleic Acids Res 1988 Dec 9; 16(23):11366.PMID: 2849765