Goat anti-Vimentin Antibody

Goat anti-Vimentin Antibody
Principle Name: Vimentin
Official Symbol: VIM
All Name and Symbols: VIM; vimentin; FLJ36605
Accession Number(s): NP_003371.2
Peptide Sequence: QVINETSQHHDDLE, is from C Terminus
Human Gene ID(s): 7431
Non-Human GeneID(s): 22352 (mouse); 81818 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, FCPeptide ELISA: antibody detection limit dilution 1 to 4000.
Western Blot: Approx 55kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60kDa band in Rat Ovary lysates (calculated MW of 53.7kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1).
Reference(s): Maraina CH, Nurdayana AK, Rusni D, Azwany Y. Diagnostic value of anti-modified citrullinated vimentin in rheumatoid arthritis. Int J Rheum Dis. 2010 Oct; 13(4):335-9.PMID: 21199468