Goat anti-ICSBP1 / IRF8 Antibody

Pep ELISA, WB, IHC

Goat anti-ICSBP1 / IRF8 Antibody
Principle Name: ICSBP1 / IRF8
Official Symbol: IRF8
All Name and Symbols: IRF8; ICSBP1; interferon consensus sequence binding protein 1; IRF-8; H-ICSBP; interferon regulatory factor 8
Accession Number(s): NP_002154.1
Peptide Sequence: QRSFFRENQQITV, is from C Terminus
Human Gene ID(s): 3394
Non-Human GeneID(s): 15900 (mouse);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 45-50kDa band seen in K562 lysate (Predicted MW of 47kDa according to NP_002154.1). Recommended concentration: 0.5-2ug/ml.
Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5ug/ml.
Reference(s): Weisz A, Marx P, Sharf R, Appella E, Driggers PH, Ozato K, Levi BZ. Human interferon consensus sequence binding protein is a negative regulator of enhancer elements common to interferon-inducible genes. J Biol Chem. 1992 Dec 15; 267(35):25589-96.PMID: 1460054