Description |
Goat anti-Tryptophan hydroxylase 2 / TPH2 Antibody Principle Name: Tryptophan hydroxylase 2 / TPH2 Official Symbol: TPH2 All Name and Symbols: TPH2; tryptophan hydroxylase 2; HGNC:20692; FLJ37295; NTPH ; neuronal tryptophan hydroxylase; ADHD7; MGC138871; MGC138872; tryptophan 5-monooxygenase 2 Accession Number(s): NP_775489.2 Peptide Sequence: NKPNSGKNDDKGNK, is from internal region Human Gene ID(s): 121278 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, IHC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 16000. Western Blot: Preliminary experiments in human brain lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). Immunohistochemistry: In paraffin embedded Human Brain Stem shows layered neuronal staining. Recommended concentration: 4-6ug/ml. Reference(s): Zhou Z, Roy A, Lipsky R, Kuchipudi K, Zhu G, Taubman J, Enoch MA, Virkkunen M, Goldman D. Haplotype-based linkage of tryptophan hydroxylase 2 to suicide attempt, major depression, and cerebrospinal fluid 5-hydroxyindoleacetic acid in 4 populations. Arch Gen Psychiatry. 2005 Oct; 62(10):1109-18. .PMID: 16203956 |