Goat anti-Tryptophan hydroxylase 2 / TPH2 Antibody

Goat anti-Tryptophan hydroxylase 2 / TPH2 Antibody
Principle Name: Tryptophan hydroxylase 2 / TPH2
Official Symbol: TPH2
All Name and Symbols: TPH2; tryptophan hydroxylase 2; HGNC:20692; FLJ37295; NTPH ; neuronal tryptophan hydroxylase; ADHD7; MGC138871; MGC138872; tryptophan 5-monooxygenase 2
Accession Number(s): NP_775489.2
Peptide Sequence: NKPNSGKNDDKGNK, is from internal region
Human Gene ID(s): 121278
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Preliminary experiments in human brain lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Immunohistochemistry: In paraffin embedded Human Brain Stem shows layered neuronal staining. Recommended concentration: 4-6ug/ml.
Reference(s): Zhou Z, Roy A, Lipsky R, Kuchipudi K, Zhu G, Taubman J, Enoch MA, Virkkunen M, Goldman D. Haplotype-based linkage of tryptophan hydroxylase 2 to suicide attempt, major depression, and cerebrospinal fluid 5-hydroxyindoleacetic acid in 4 populations. Arch Gen Psychiatry. 2005 Oct; 62(10):1109-18. .PMID: 16203956