Goat anti-ERG Antibody

Pep ELISA, WB

Goat anti-ERG Antibody
Principle Name: ERG
Official Symbol: ERG
All Name and Symbols: ERG; v-ets erythroblastosis virus E26 oncogene like (avian); HGNC:3446; erg-3; p55; transcriptional regulator ERG (transforming protein ERG); v-ets avian erythroblastosis virus E26 oncogene related; v-ets erythroblastosis virus E26 oncogene like; v-ets er
Accession Number(s): NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1|
Peptide Sequence: PNTRLPTSHMPSH, is from C Terminus
Human Gene ID(s): 2078
Non-Human GeneID(s): 13876 (mouse); 170909 (rat);
Important Comments: This antibody is expected to recognise all reported isoforms (NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 55kDa band observed in lysates of cell line MOLT4 (calculated MW of 53.8kDa according to NP_891548.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.