Goat anti-ERG Antibody

Goat anti-ERG Antibody
Principle Name: ERG
Official Symbol: ERG
All Name and Symbols: ERG; v-ets erythroblastosis virus E26 oncogene like (avian); HGNC:3446; erg-3; p55; transcriptional regulator ERG (transforming protein ERG); v-ets avian erythroblastosis virus E26 oncogene related; v-ets erythroblastosis virus E26 oncogene like; v-ets er
Accession Number(s): NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1|
Peptide Sequence: PNTRLPTSHMPSH, is from C Terminus
Human Gene ID(s): 2078
Non-Human GeneID(s): 13876 (mouse); 170909 (rat);
Important Comments: This antibody is expected to recognise all reported isoforms (NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 55kDa band observed in lysates of cell line MOLT4 (calculated MW of 53.8kDa according to NP_891548.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.
Reference(s): Tomlins SA, Rhodes DR, Perner S, Dhanasekaran SM, Mehra R, Sun XW, Varambally S, Cao X, Tchinda J, Kuefer R, Lee C, Montie JE, Shah RB, Pienta KJ, Rubin MA, Chinnaiyan AM. Recurrent fusion of TMPRSS2 and ETS transcription factor genes in prostate cancer. Science. 2005 Oct 28; 310(5748):644-8. .PMID: 16254181