Description |
Goat anti-NEDD1 Antibody Principle Name: NEDD1 Official Symbol: NEDD1 All Name and Symbols: NEDD1; neural precursor cell expressed, developmentally down-regulated 1 Accession Number(s): NP_690869.1 Peptide Sequence: NQTRNSEKFEKPEN, is from internal region Human Gene ID(s): 121441 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 32000. Western Blot: Approx 75kDa band observed in lysates of cell line HeLa (calculated MW of 72.0kDa according to NP_690869.1). Recommended concentration: 1-3ug/ml. An additional band was also consistently observed at 37kDa. This band was successfully blocke Reference(s): Haren L, Remy MH, Bazin I, Callebaut I, Wright M, Merdes A. NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly. J Cell Biol. 2006 Feb 13; 172(4):505-15. Epub 2006 Feb 6. .PMID: 16461362 |